ISSN 0439-755X
CN 11-1911/B
主办：中国心理学会
　　　中国科学院心理研究所
出版：科学出版社

心理学报 ›› 2000, Vol. 32 ›› Issue (2): 210-216.

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CNTF对应激大鼠行为障碍和海马CA1神经元损害的作用

严进;路长林;汤淑萍;何成;王成海;王雪琦;黄爱军;孟玲;鲍璇;张铁峰   

  1. 第二军医大学基础部神经生物学教研室,第二军医大学基础部神经生物学教研室,第二军医大学基础部组织胚胎学教研室!上海200433,第二军医大学基础部神经生物学教研室,第二军医大学基础部神经生物学教研室,第二军医大学基础部神经生物学教研室,第二军医大学基础部神经生物学教研室
  • 出版日期:2000-06-25 发布日期:2000-06-25
  • 通讯作者: 严进

EFFECTS OF CILIARY NEUROTROPHIC FACTOR ON BEHAVIORAL DISORDER AND HIPPOCAMPAL CA1 NEURONAL DAMAGE INDUCED BY STRESS IN RATS

Yan Jin ;Lu Changlin; Tang Shuping (Dept. of Neurobiology, Second Military Medical University, Shanghai 200234)   

  • Published:2000-06-25 Online:2000-06-25

被引次数

24

摘要： 实验采用 ｏｐｅｎ ｆｉｅｌｄ测定、 Ｎｉｓｓｌ染色、 Ｂｉｅｌｓｃｈｏｗｓｋｙ－Ｇｒｏｓ－Ｌａｗｒｅｎｔｊｅｗ染色和常规透射电镜技术，观察急性和慢性足底电击应激大鼠的ｏｐｅｎ ｆｉｅｌｄ行为和海马ＣＡ１神经元形态的变化，及双侧海马注射睫状神经营养因子（ＣＮＴＦ）对它的影响。结果表明，急性应激大鼠ｏｐｅｎ ｆｉｅｌｄ行为活动增加，海马ＣＡ１神经元形态无明显变化；慢性应激大鼠ｏｐｅｎ ｆｉｅｌｄ行为活动减少，海马ＣＡ１神经元出现明显的损伤性形态变化；睫状神经营养因子对对照组大鼠和急性应激大鼠的ｏｐｅｎ ｆｉｅｌｄ行为和海马ＣＡ１神经元形态均无明显作用，但可显著减轻慢性应激大鼠海马ＣＡ１神经元损伤程度，改善其行为障碍。实验结果提示睫状神经营养因子可能通过保护海马神经元从而改善慢性应激大鼠的行为障碍。

关键词: 睫状神经营养因子, 应激, 行为, 海马CA1神经元

Abstract: Objective The experiment was to investigate into the effects of ciliary neurotrophic factor (CNTF) on behavioral disorder and hippocampal CA1 neuronal damage induced by stress in rats. Methods The footshock stress-induced changes of behaviors and morphology of hippocampal CA1 neurons during acute or chronic stress in tats, and the effects of CNTF administrated into bilateral hippocampus on the changes were observed with open field test Nissl stain, Bielschowsky-Gros-Lawrentjew stain and electron microscope. Results The open field behaviors increased and morphology of hippocampal CAI neurons did not change during acute stress in rats. The open field behaviors decreased and hippoampal CA1 neuronal damage occurred during chronic stress. CNTF administrated into bilateral hippocampus had no effect on behavior and morphology of hippocampal CA1 neurons in control and acute stressful rats, but it significantly reduced hippocampal CA1 neuronal damage and improved behavioral disorder induced by chronic stress. Conclusion CNTF could improve depressive behavior induced by chronic stress via protection of hippocampal CA1 neurons.

Key words: ciliary neurotrophic factor, stress, behavior, hippocampal CA1 neuron Other authors are omitted