Humility is a traditional virtue esteemed in Chinese culture, exerting positive effects on individuals, organizations, and society. Combining qualitative research, corpus compilation, and a review of previous scales, this study constructed the structure of the humility trait within Chinese culture and developed the Chinese Humility Scale. The scale comprises five dimensions: Accurate Self-Perception, Other-Enhancement, Dialectical Thinking, Self-Effacement, and Self-Improvement. Based on this five-dimensional structure, Latent Profile Analysis was utilized, revealing five distinct profiles of humility manifestations in Chinese culture: “Humble,” “Moderately Humble,” “Overly Humble,” “Unhumble,” and “Strategically Humble.” This research clarifies the conceptual connotations of humility within Chinese culture and provides a novel measurement tool for future empirical studies, as well as for the identification and selection of well-rounded talents possessing both competence and humility. Furthermore, it plays a positive role in inheriting and promoting the traditional virtue of humility while facilitating individual development and social harmony.