Abstract: To establish the model of conditioned enhancement of antibody production. Forty-nine male and adult Wistar rats were used. A novel taste of saccharin was served as the conditioned stimulus (CS) and an injection of a protein antigen ovalbumin as the unconditioned stimulus (UCS). After a CS/UCS pairing was made, animals were re-exposed to the CS alone. Blood samples were periodically collected by tail incisions after re-exposure to the CS. The level of anti-OVA antibody production was measured by enzyme linked immunosorbent assay (ELISA). The results showed that the levels of anti-OVA antibody production of the group CS were significantly greater than the controls at the day 15, 20 and 25 after the CS presentation. The pattern of the conditioned anti-OVA IgG production was similar to that of the primary antibody response. The results reinforced the view that the enhancement of antibody production can be induced by single-trial Pavlovian conditioning where the CS alone is sufficient.

Key words: conditioned immunoenhancement, dynamic analysis, rat, ovalbumin