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THE PLASMA CORTICOSTERONE IN RATS DURING PHYSICAL AND PSYCHOLOGICAL STRESS
Yan Jinl,Wang Chunan,Ye Ali,Chen Yizhang Laboratory of Neuroscience, Department of Physiology, The Second Military Medical University, Shanghai, China, 200433
1991, 23 (4):
84-91.
The plasma corticosterone level of rats was determined and used as acriterion for magnitude of stress response in this model. The rats wererandomly divided into three groups: C, control group; R, regular lightgroup; Ⅰ, irregular light group.The experiment was successively carried out in three stages:Ⅰ. adaptation (7 days); Ⅱ. development of stress(7 days);and Ⅲ. psychological induction of stress (0-24 hrs). Stage Ⅰ: all animalswere adapted to the cage and other environmental cues. Stage Ⅱ. in groupR electric shocks were applied to the tail regularly with a fixed intervalafter the lights were given. In group I the same amount of light and ele-ctric shocks were irregularly applied. In group C only irregular light wasgiven. Stage Ⅲ. all tail shocks were omitted and the same protocol oflights were used in different groups. The results showed that the plasmacorticosterone levels in the R and I groups were significantly higher thanthose in the C group (p<0.01.StageⅡ: R,24.9+\-5.8; Ⅰ,34.9+\-2.0;C,11.7+\-1.7. Stage Ⅲ. R,24.1+\-3.3; Ⅰ, 33.9+\-2.5; C,13.1+\-5.0. In every group n was 9 and the unit was ug/dl.), there was a signi-ficant difference between stress responses of R and I groups, both at stageⅡ and Ⅲ (P<0.05).
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