ISSN 0439-755X
CN 11-1911/B
主办:中国心理学会
   中国科学院心理研究所
出版:科学出版社

心理学报 ›› 1991, Vol. 23 ›› Issue (1): 90-95.

• • 上一篇    下一篇

抗精氨酸加压素单克隆抗体对小鼠行为的影响

张艳萍;张世仪;张敏磊;孙汇桥;黄醒亚   

  1. 中国医学科学院基础医学研究所,中国医学科学院基础医学研究所,中国医学科学院基础医学研究所,中国医学科学院基础医学研究所,中国医学科学院基础医学研究所 北京 ,北京 ,北京 ,北京 ,北京 国家自然科学基金委员会
  • 出版日期:1991-03-25 发布日期:1991-03-25
  • 通讯作者: 张艳萍

THE BEHAVIORAL EFFECT OF A MONOCLONAL ANTIBODY TO [ARG~8] VASOPRESSIN INJECTED CENTRALLY OR PERIPHERALLY IN MICE

Zhang Yanping;Zhang Shiyi;Zhang Minlei;Sun Huiqiao;Huang Xingya Institute of Basic Medical Sciences, Chinese Academy of Medical sciences, Beijing   

  • Published:1991-03-25 Online:1991-03-25

摘要: 本工作在我们已报道的外源性精氨酸加压素([Arg~8] Vasoprpssin,AVP)促进小鼠记忆巩固结果的基础上,应用本实验室研制成功的抗精氨酸加压素单克隆抗体(Monoclonal Antibody to[Arg~8]Vasopressin,AVPMcAb)中枢及外周注射,观察小鼠内源性AVP对记忆巩固的影响。结果为:中枢注射AVP-McAb1.5—4.5毫微克/鼠后,小鼠抑制性回避反应的潜伏期和生理盐水对照组相比,均显著缩短(P<0.05);而外周尾静脉注射AVPMcAb0.15—1.50微克/鼠对小鼠此行为反应无影响,与生理盐水组对比,潜伏期无显著性差异。这提示,中枢内源性生理水平的加压素可能对小鼠记忆巩固起易化作用,而外周的加压素在生理条件下可能无此效应。 小鼠游泳试验中,训练后立即中枢注射AVPMcAb也降低小鼠游泳平衡的反应。这暗示,中枢内源性加压素在该行为模式上也参与记忆的调制过程。

关键词: NULL

Abstract: Previously the authors reported on peripheral or central injection ofAVP facilitated memory retention in an inhibitory avoidance (IA) taskin mice. In the present study, we investigated the behavioral effect ofendorgenous vasopressin by using Monoclonal Antibody to [Arg~8] vasopr-essin (AVPMcAb), which was made by another group in the same lab-oratory injected centrally (icy) or peripherally (iv). Male Kunming mice were used in two sets of behavioral experiments,one set was IA task, another was Swimming Test (ST). The retentionlatency (in IA) or percentage of hindlegs immobile time (in ST) wasrespectively recorded as a measure of behavior retention. Results. 1) immediately after IA training, administration (icv)of AVPMcAb (1.5 and 4.5 ng/mouse) impaired retention (p<0.05). 2)AVPMeAb (0.15-1.50 μg/mouse) given iv injection immediately after I Atraining, had no influence on retention (p>0.05). 3) The behavior ret-ention of ST was depressed by AVPMcAb (4.5 ng/mouse) injection(icv). The data suggests that the central but not peripheral endorgenous va-sopressin might have some physiological influence on the animals' memoryfacilitation.

Key words: NULL