ISSN 1671-3710
CN 11-4766/R
主办：中国科学院心理研究所
出版：科学出版社
2025年4月25日 星期五

心理科学进展 ›› 2023, Vol. 31 ›› Issue (suppl.): 132-132. cstr: 32111.14.2023.00132

• 临床与转化视觉 • 上一篇    下一篇

Restoring Antagonistic Center-surround Receptive Field in Blind Mice by a Silicon Photodiode-based Visual Prosthesis

Tianyun Zhanga, Ruyi Yanga, Peng Zhaoa, Lizhu Lib, Chen Pengd, Gengfeng Zhengd, Aihua Chenc, Xing Shengb, Biao Yana   

  1. aJiayi Zhangaa State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institutes of Brain Science, Institute for Medical and Engineering Innovation, Department of Ophthalmology and Vision Science, Eye & ENT Hospital, Fudan University, Shanghai 200032, China;
    bDepartment of Electronic Engineering, Center for Flexible Electronics Technology, Tsinghua University, Beijing, China;
    cKey Laboratory of Brain Functional Genomics, East China Normal University, Shanghai 200062, China;
    dLaboratory of Advanced Materials, Department of Chemistry, Fudan University, Shanghai 200240, China
  • 出版日期:2023-08-26 发布日期:2023-09-08

Restoring Antagonistic Center-surround Receptive Field in Blind Mice by a Silicon Photodiode-based Visual Prosthesis

Tianyun Zhanga, Ruyi Yanga, Peng Zhaoa, Lizhu Lib, Chen Pengd, Gengfeng Zhengd, Aihua Chenc, Xing Shengb, Biao Yana   

  1. aJiayi Zhangaa State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institutes of Brain Science, Institute for Medical and Engineering Innovation, Department of Ophthalmology and Vision Science, Eye & ENT Hospital, Fudan University, Shanghai 200032, China;
    bDepartment of Electronic Engineering, Center for Flexible Electronics Technology, Tsinghua University, Beijing, China;
    cKey Laboratory of Brain Functional Genomics, East China Normal University, Shanghai 200062, China;
    dLaboratory of Advanced Materials, Department of Chemistry, Fudan University, Shanghai 200240, China
  • Online:2023-08-26 Published:2023-09-08

Abstract: PURPOSE: Retinal degeneration disease (RDD) has become the largest cause of irreversible blindness by year 2020. Retinal prosthetics was one of the most fruitful approaches to combat such disease, but bottom-up inhibitory signal, which was at the center of antagonistic center-surround receptive field and fine visual features, has rarely been considered in the development of retinal prosthetics so far. This study aims to generate artificial antagonistic center-surround receptive fields in blind retina by aligning inhibitory and excitatory silicon photodiodes into receptive field-like patterns, and to evaluate the electrophysiological and behavioral performances of such design.
METHODS: Subcellular structural changes on the neuro-material surface were quantified by IHC staining and 3-D cell reconstruction. Tests on the capabilities of both excitatory (Si np+) and inhibitory (Si pn+) silicon materials are conducted by ex vivo retina patch clamp. Excitatory and inhibitory cellular responses by photoelectrical stimulations were also recorded by ex vivo retina patch clamp, with inhibitory responses recorded under off-BC pre-excitation via KA administration.
RESULTS: RGC and RBC survival were not affected by implantation duration. Strengthened dendritic trunk-like structure and multiplied dendritic tips and bifurcations have been observed on the RBCs from the neuro-material interface. Both types of Si materials showed light intensity-dependent photovoltaic responses, and can elicit excitatory or inhibitory responses on RGCs.
CONCLUSIONS: Preliminary results suggest minor biocompatibility issue and possible RBC neurite proliferation upon prosthesis integration. Feasibility of restoring center-surround antagonism by excitatory plus inhibitory materials is validated.

Key words: retinal degeneration disease, retinal prosthesis, center-surround antagonism, silicon photodiode, stimulation-induced neuroplasticity