ISSN 0439-755X
CN 11-1911/B
主办：中国心理学会
　　　中国科学院心理研究所
出版：科学出版社

心理学报 ›› 1998, Vol. 30 ›› Issue (2): 187-192.

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视剥夺及视剥夺学习后Jun样蛋白在雏鸡两侧半球中不同表达的研究

高杨;胡家芬;管林初;匡培梓;姜树军;匡培根   

  1. 中国科学院心理研究所，中国人民解放军总医院神经介质实验室
  • 出版日期:1998-06-25 发布日期:1998-06-25
  • 通讯作者: 高杨

EXPRESSION OF JUN-LIKE PROTEIN IN THE HEMISPHEREOF CHICK FOLLOWING MONOVISUAL DEPRIVATION ANDONE-TRIAL PASSIVE AVOIDANCE TASK

Gao Yang Hu Jiafen Guan Linchu Kuang Peizi (Institute of Psychology Chinese Academy of Sciences,Beijing,100012) Jiang Shujun Kuang Peigen (Neurotransmitter Research Laboratory , Chinese PLA General Hospital ,Beijing,100853)   

  • Published:1998-06-25 Online:1998-06-25

摘要： 该实验利用免疫组化技术，在已有实验的基础上进一步观察比较了左眼视剥夺及单眼学习后，Ｊｕｎ样蛋白在雏鸡两侧半球ＨＶ和ＬＰＯ中表达的差异。实验结果表明：１．视剥夺２、２４、４８、７２小时后观测到Ｊｕｎ样蛋白在左、右半球ＨＶ和ＬＰＯ中的表达逐渐增高，并且于视剥夺４８小时表达到达峰值。同时仅在视剥夺２小时后观测到两半球ＬＰＯ中Ｊｕｎ样蛋白的表达存在差异，在其余各视剥夺时程中两侧半球的ＬＰＯ、ＨＶ中Ｊｕｎ样蛋白的表达均无显著差异。２．视剥夺２小时和２４小时后均在学习后７０分钟组中观测到Ｊｕｎ样蛋白在左、右半球的ＨＶ和ＬＰＯ中表达的显著升高，而在视剥夺２小时学习后１０分钟只观测到左半球ＨＶ和ＬＰＯ中Ｊｕｎ样蛋白表达的显著升高，在视剥夺２４小时学习后１０分钟中仅观测到左半球ＬＰＯ中Ｊｕｎ样蛋白的显著升高。同时也仅在视剥夺２小时学习后１０分钟观测到两侧半球ＨＶ、ＬＰＯ中Ｊｕｎ样蛋白表达的显著性差异。３．无论单纯视剥夺组还是单眼学习组，各组同侧半球中ＬＰＯＪｕｎ样蛋白的表达显著高于ＨＶ的Ｊｕｎ样蛋白的表达现象在视剥夺４８小时后消失。

关键词: 雏鸡, Jun样蛋白, 免疫组化技术, HV, LPO, 一次性味觉厌恶回避学习

Abstract: Immunohistochemical technique was used in the present study to detect and compare Jun expressed in HV and LPO of the chick ' s two hemispheres following monovisual deprivation and one-trial passive avoidance task. It was shown that monovisual deprived 2 hours, 24 hours, 48 hours or 72 hours induced Jun expression definitely increased in HV and LPO of the two hemispheres, and Jun expression came to the highest level when visually deprived 48 hours; the expression in the left LPO was higher than that in the right LPO after being visually deprived 2 hours; monovisually deprived 2 hours and 24 hours following one-trail passive avoidance task and memory retention test at 70 minute after learning induced Jun expression to continue increase in HV and LPO of the two hemispheres; monovisually deprived 2 hours test at 10 minute just induced Jun expression obvious increase in the left LPO and HV; only the left LPO showed high level Jun expression after monovisually deprived 24 hours and test at 10 minutes; in the same hemisphere, the fact that the Jun expression of LPO was higher than that in HV disappeared after being visually deprived 48 hours.

Key words: chick, Jun-like protein, Immunohistochemical, hemisphere, HV, LPO one-trail passive avoidance task