ISSN 0439-755X
CN 11-1911/B
主办：中国心理学会
　　　中国科学院心理研究所
出版：科学出版社

心理学报 ›› 1979, Vol. 11 ›› Issue (3): 84-89.

• • 上一篇    下一篇

记忆与学习的脑化学研究 Ⅱ.海马内注射胰蛋白酶与胰凝乳蛋白酶对大白鼠暗箱迴避模式记忆的影响

罗胜德;李德明;孙丽华   

  1. 中国科学院心理研究所 ,中国科学院心理研究所 ,中国科学院心理研究所
  • 发布日期:1979-09-25 出版日期:1979-09-25
  • 通讯作者: 罗胜德

BIOCHEMICAL STUDIES OF MEMORY AND LEARNING Ⅱ.INFLUENCE OF HIPPOCAMPUS INJECTION OF PANCREATIC TRYPSIN AND CHYMOTRYPSIN ON MEMORY OF DARK BOX AVOIDANCE(DBA)TASK IN ALBINO RATS

Lo Sheng-de;Li De-ming;Sun Li-hua Institute of Psychology, Academia Sinica   

  • Online:1979-09-25 Published:1979-09-25

摘要： 本实验选择了胰凝乳蛋白酶与胰蛋白酶,它们同属水解酶,能够内切蛋白质与肽类底物的某些键位。26只刚成年的雄性大鼠分作三组:生理盐水/不巩固组(NS/NC)、胰蛋白酶/不巩固组(T/NC)与胰凝乳蛋白酶/不巩固组(CT/NC)。 实验结果表明,双侧海马内注射(AP_2L_2H_4与AP_4L_3H_5)胰凝乳蛋白酶与胰蛋白酶后,对暗箱廻避(DBA)模式记忆有不同的影响。胰蛋白酶注射后,该记忆保持明显降低,而胰凝乳蛋白酶注射后仍可巩固。所用的实验剂量大致为半致死剂量的三分之一。

关键词: NULL

Abstract: Two instrumental enzymes, Chymotrypsin (CT) and Trypsin (T), both are pro-teolytic enzymes capable of destroying some bonds of proteins and peptides, were usedin this experiment. A total of 26 young adu1t male albino rats served as the threenon-consolidation groups (NC) of DBA memory: normal saline solution (NS/NC),Trypsin (T/NC) and Chymotrypsin (CT/NC). After bilateral injection of CT and T into the Hippocampus (iH: AP_2L_2H_4 andAP_4L_3H_5), different effects were observed on the conditioned reflex of DBA memorymodel (CR_(DBA)). 72 hours after iH. T, retention of CR_(DBA) clearly decreased, but afteriH. CT, memory remains consolidated. Differences in successive number of CR_(DBA)(SN_(CR)) and rate of CR_(DBA) (CR%) were statistically significant. The experimentaldoses of iH. T and CT approximately equal 1/3 of their LD_(50).

Key words: NULL