ISSN 1671-3710
CN 11-4766/R
主办：中国科学院心理研究所
出版：科学出版社

心理科学进展 ›› 2023, Vol. 31 ›› Issue (suppl.): 139-139.

• 视觉功能障碍与康复 • 上一篇    下一篇

Artificial Photoreceptors Based on Au@TiO2-x Nanowire Arrays Restore Visual Function in Blind Mice and Monkeys

Ruyi Yanga, Peng Zhaoa, Liyang Wangc, Chenli Fengc, Chen Pengb, Xingdong Chena, Gengfeng Zhengb, Chunhui Jianga,*, Yuanzhi Yuanc,*, Biao Yana,*, Jiayi Zhanga,*   

  1. aState Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institutes of Brain Science, Institute for Medical and Engineering Innovation, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai 200032, P.R. China;
    bLaboratory of Advanced Materials, Department of Chemistry, Faculty of Chemistry and Materials Science, Fudan University, Shanghai 200240, P.R. China;
    cDepartment of Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China
  • 出版日期:2023-08-26 发布日期:2023-09-08

Artificial Photoreceptors Based on Au@TiO2-x Nanowire Arrays Restore Visual Function in Blind Mice and Monkeys

Ruyi Yanga, Peng Zhaoa, Liyang Wangc, Chenli Fengc, Chen Pengb, Xingdong Chena, Gengfeng Zhengb, Chunhui Jianga,*, Yuanzhi Yuanc,*, Biao Yana,*, Jiayi Zhanga,*   

  1. aState Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institutes of Brain Science, Institute for Medical and Engineering Innovation, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai 200032, P.R. China;
    bLaboratory of Advanced Materials, Department of Chemistry, Faculty of Chemistry and Materials Science, Fudan University, Shanghai 200240, P.R. China;
    cDepartment of Ophthalmology, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China
  • Online:2023-08-26 Published:2023-09-08

Abstract: PURPOSE: Photoreceptor degeneration is the main cause of blindness in retinal degenerative diseases such as retinitis pigmentosa and age-related macular degeneration. The objective of this study is to assess the performance of Au@TiO2-x nanowire arrays as artificial photoreceptors to restore primary visual function in blind mice and non-human primates with photoreceptor degeneration.
METHODS: To evaluate the improvement in visual temporal and spatial resolution in blind mice, Au@TiO2-x nanowire arrays were assessed using in vitro patch-clamp recordings and mouse behavior paradigm based on 2AFC. Long-term continuous monitoring of light-induced responses in the visual cortex neurons of blind mice after implantation of nanowire arrays was conducted using two-photon calcium imaging technology. The safety, stability, and light-responsive capabilities of monkeys implanted with nanowire arrays were examined through fundus imaging, OCT imaging, and visually guided saccade behavior experiments, respectively.
RESULTS: Subretinal implantation of nanowire arrays in blind mice was capable of detecting drifting gratings and flashing objects at low light intensity thresholds (15.70 - 18.09 μW·mm-2), with spatial and temporal resolutions of 77.5 μm and 3.92 Hz, respectively. Additionally, long-term in vivo calcium imaging suggested plastic changes in visual cortical circuits after nanowire implant. The nanowire arrays exhibited good biocompatibility and stability for 54 weeks after subretinal implantation in monkeys, capable of detecting light stimuli of 0.5º in diameter at 10 μW·mm-2 in visually guided saccade experiments.
CONCLUSIONS: Our findings demonstrate the potential of Au@TiO2-x nanowire array as artificial photoreceptors to ameliorate visual deficits in patients with photoreceptor degeneration.

Key words: Retina, Artificial photoreceptors, Retinal prosthesis, Nanowire array