ISSN 1671-3710
CN 11-4766/R

Advances in Psychological Science ›› 2016, Vol. 24 ›› Issue (Suppl.): 18-.

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The role of retinal dopamine in C57BL/6 mouse refractive development as revealed by intravitreal administration of 6-hydroxydopamine

Xiao-Hua Wu; Kang-Wei Qian; Guo-Zhong Xu; Yun-Yun Li; Yuan-Yuan Ma; Furong Huang; Yan-Qing Wang; Xiangtian Zhou; Jia Qu; Xiong-Li Yang; Yong-Mei Zhong; Shi-Jun Weng   

  1. Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, China, 200032
    Department of Integrative Medicine and Neurobiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China, 200032
    School of Ophthalmology and Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China, 325027
  • Online:2016-12-31 Published:2016-12-31


PURPOSE: While retinal dopamine (DA) has been long implicated in myopia development, current studies demonstrate that retinal DA levels are unaltered in C57BL/6 mice with form-deprivation myopia. This work was undertaken to explore whether and how refractive development is perturbed in this mouse strain when retinal DA levels are reduced by 6-hydroxydopamine (6-OHDA) administration.
METHODS: 6-OHDA of different doses was intravitreally applied. Retinal DA levels were measured by HPLC and TH levels analyzed by quantitative Western blotting. To choose appropriate 6-OHDA doses that significantly reduce retinal DA levels, but caused minimal disturbance of overall retinal physiology, ERG analysis was performed. Refractive errors were measured using a photorefractor, and ocular biometry assessed with optical coherence tomography and photokeratometry.
RESULTS: 6-OHDA of 6.25 μg and 12.5 μg significantly reduced retinal levels of DA and TH, but did not significantly affect ERG a- and b-wave amplitudes. With normal visual experience, 6-OHDA induced myopic refractive shifts in a dose-dependent fashion. Form-deprivation induced further myopic shifts in 6-OHDA injected eyes, but did not cause further decline in retinal DA. Furthermore, 6-OHDA administration resulted in a shorter axial length and a steeper cornea, while form-deprivation led to a longer axial length, without changing the corneal radius of curvature.
CONCLUSIONS: Reducing retinal DA levels by 6-OHDA led to myopic refractive shifts in C57BL/6 mice, which mainly resulted from a steeper cornea. We propose that, in addition to the DA-independent mechanism for form-deprivation induced myopic refractive shifts, there is a DA-dependent mechanism in parallel that underlies myopic refractive shifts in this mouse strain.

Key words: refractive development, form-deprivation myopia, dopamine, C57BL/6 mouse, 6-hydroxydopamine