Abstract: Using anisodine to prepare animal models of learning and memory disorder with water maze test, step clown test and step through test in mice was intro- duced in this paper. The results showed, anisodine (10 mg/kg)could significantly induce learning disorder in mice. Time through all pathways in the water maze was prolonged and errors in passing the blind alleys obviously increased. Anis- odine(10mg/kg)could significantly produce mempry dysfunction in mice: the latencies of the step down test and step through test were significantly shorten- ed, and errors of response in the step down test and step through test increased. The results futher indicated that the step through test is more sensitive than the step down test for testing memory dysfunction in mice.

Key words: anisodine, learning and memory, water maze test, step down test, step through test, animal model