Please wait a minute...
   2012, Vol. 44 Issue (4) : 459-468     DOI:
|
Upregulation of K+ Current in Hippocampal Neurons from Rat with Depressive-Like Behavior Induced by Lipopolysaccharide
CHI Shao-Peng;QI Zhi;JI Guang-Ju;KUANG Xue-Ying;LIN Wen-Juan
(1Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China)
(2Graduate School of the Chinese Academy of Sciences, Beijing 100039, China)
(3National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China)
(4Department of Physiology, Medical College of Xiamen University, Xiamen 361005, China)
Download: PDF(683 KB)  
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks    
Abstract  Lipopolysaccharide (LPS)-induced immunity activation can result in evident depressive-like behavior, such as anhedonia and reduced locomotion. Earlier studies have shown that K+ channels, such as Kv7 and TREK-1, are involved in the development of depressive-like behavior in animal models. In the present study, we show that LPS (200 μg/kg) could induce significant short-term depressive-like behavior in rat. In order to get insight into the underlying molecular mechanism, we investigated the potential involvement of rat hippocampal neuron voltage-dependent K+ channels in the depressive-like behaviors induced by LPS. Methods: Twenty rats were randomly divided into LPS and control groups, each with ten rats. The rats in the LPS group were injected with LPS (200 μg/kg), while saline was injected in the rats of the control group. The saccharin preference test and open field test were carried out 2 hours and 24 hours after injection of LPS to assess short-term or long-term effects on animal behavior, respectively. Next, 12 rats were randomly divided into LPS 2 hr, LPS 24 hr and control groups, each with four rats for assessing the effect of LPS on K+ channel currents of hippocampal neurons by using whole-cell patch-clamp configuration. Results: The data showed that LPS induced depressive-like behavior in rat after 2 hours of single injection. However, this depressive-like behavior was recovered after 24 hours of single LPS injection. In agreement with these behavioral observations, voltage-dependent K+ current density was increased in neurons isolated from rat after 2 hours of single LPS injection, whereas no significant change was found after 24 hours injection. Conclusion: Our results demonstrate that LPS–induced immunity activation can result in evident short term depressive–like behavior in rats, which are coincident with LPS induced up-regulation of voltage-dependent K+ current in rat hippocampal neurons. This result suggests that voltage-dependent K+ current might contribute to the development of the depressive-like behavior induced by LPS.
Keywords lipopolysaccharide      depressive-like behavior      hippocampal neuron      voltage-dependent potassium channel     
Corresponding Authors: LIN Wen-Juan   
Issue Date: 28 April 2012
Service
E-mail this article
E-mail Alert
RSS
Articles by authors
CHI Shao-Peng
QI Zhi
JI Guang-Ju
KUANG Xue-Ying
LIN Wen-Juan
Cite this article:   
CHI Shao-Peng,QI Zhi,JI Guang-Ju, et al. Upregulation of K+ Current in Hippocampal Neurons from Rat with Depressive-Like Behavior Induced by Lipopolysaccharide[J]. , 2012, 44(4): 459-468.
URL:  
http://journal.psych.ac.cn/xlxb/EN/     OR     http://journal.psych.ac.cn/xlxb/EN/Y2012/V44/I4/459
[1] TANG Mingming;PAN Yuqin;LIN Wenjuan. A New Animal Model of Depression Induced by Repeated Central Lipopolysaccharide Administration[J]. Acta Psychologica Sinica, 2014, 46(5): 639-646.
[2] PAN Yu-Qin,LIN Wen-Juan,WANG Dong-Lin,SUN Han. The Effect of Lipoposaccharide on Depressive-like Behavior Induced by Forced Swim Stress[J]. , 2012, 44(3): 322-329.
[3] KUANG Xue-Ying,LIN Wen-Juan,WANG Dong-Lin,QI Xiao-Li,PAN Yu-Qin,SUN Han,XIE Xi. The Effect of Cholecystokinin Octapeptide on Lipopolysaccharide-induced Sickness Behavior and Peripheral Cytokine Secretion[J]. , 2011, 43(03): 283-293.
[4]

Pan Yuqin,Wang Donglin,Lin Wenjuan

. Animal Research on Immunity Activation-Induced Depressive-Like Behavior: Doses and Time Effects[J]. , 2007, 39(06): 1041-1047.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
Copyright © Acta Psychologica Sinica
Support by Beijing Magtech