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   2011, Vol. 43 Issue (03) : 283-293     DOI:
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The Effect of Cholecystokinin Octapeptide on Lipopolysaccharide-induced Sickness Behavior and Peripheral Cytokine Secretion
KUANG Xue-Ying;LIN Wen-Juan;WANG Dong-Lin;QI Xiao-Li;PAN Yu-Qin;SUN Han;XIE Xi
Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China
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Abstract  Cytokines have been considered to be involved in the pathology of sickness behavior, but the role of peripheral cytokines in the pathology of sickness behavior is still elusive. Cholecystokinin octapeptide (CCK-8) has been proved to inhibit the lipopolysaccharide (LPS)-induced secretion of peripheral proinflammatory cytokines in rats. Aim: To investigate the role of peripheral cytokines in the pathology of sickness behavior. Methods: The experiment included two parts. In Experiment 1, forty-six rats were randomly divided into 4 groups: control, LPS, CCK-40+LPS and CCK-40, each group comprised twelve rats except ten rats for the control group, and in Experiment 2 forty-eight rats were randomly divided into six groups: control, LPS, CCK-20+LPS, CCK-40+LPS, CCK-80+LPS, CCK-120+LPS, each group comprised eight rats. The CCK groups were injected with different dosages of CCK-8 solution (20μg/kg, 40μg/kg, 80μg/kg and 120μg/kg doses respectively, i.p.) half an hour before the injection of LPS (200μg/kg, i.p.), and then 2 hours after the LPS injection, the sickness behavior was measured in behavioral experiments (including the sugar-water consumption test, open field test, and elevated plus maze test), and the cytokines in serum were analyzed by immunoassays. Results: LPS induced sickness behavior: LPS group consumed less sugar water and exhibited less activities in open field test and elevated plus maze test when compared with the controls; LPS also significantly increased cytokine production including IL-1β, IL-6 and TNF-α. However, the LPS-induced sickness behavior could not be attenuated by CCK-8, though CCK-8 significantly inhibited the LPS-induced proinflammatory cytokines of IL-1β, IL-6 and TNF-α compared to the LPS group. Conclusion: The inhibition of the secretion of peripheral proinflammatory cytokines can not antagonize the lipopolysaccharide-induced sickness behavior. The peripheral proinflammatory cytokines might be not directly responsible for the sickness behavior. The cytokines acting in brain may be more related to the sickness behavior, which needs further investigation.
Keywords Cholecystokinin octapeptide      Lipopolysaccharide      Interleukin-1β      Interleukin-6      Interleukin-10      Tmor necrosis factor-α      Sickness behavior     
Corresponding Authors: LIN Wen-Juan   
Issue Date: 30 March 2011
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KUANG Xue-Ying
LIN Wen-Juan
WANG Dong-Lin
QI Xiao-Li
PAN Yu-Qin
SUN Han
XIE Xi
Cite this article:   
KUANG Xue-Ying,LIN Wen-Juan,WANG Dong-Lin, et al. The Effect of Cholecystokinin Octapeptide on Lipopolysaccharide-induced Sickness Behavior and Peripheral Cytokine Secretion[J]. , 2011, 43(03): 283-293.
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http://journal.psych.ac.cn/xlxb/EN/      OR     http://journal.psych.ac.cn/xlxb/EN/Y2011/V43/I03/283
[1] TANG Mingming;PAN Yuqin;LIN Wenjuan. A New Animal Model of Depression Induced by Repeated Central Lipopolysaccharide Administration[J]. Acta Psychologica Sinica, 2014, 46(5): 639-646.
[2] CHI Shao-Peng,QI Zhi,JI Guang-Ju,KUANG Xue-Ying,LIN Wen-Juan. Upregulation of K+ Current in Hippocampal Neurons from Rat with Depressive-Like Behavior Induced by Lipopolysaccharide[J]. , 2012, 44(4): 459-468.
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