Please wait a minute...
Acta Psychologica Sinica
|
Orbitofrontal Cortex 5-HT1A Receptor Modulate Glutamate and GABA in Depression Induced by Chronic Unpredictable Mild Stress
LI Jiangna; AN Shucheng; LI Zhen
(College of Life Science, Shaanxi Normal University, Xi’an 710119, China)
Download: PDF(1435 KB)   Review File (1 KB) 
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks    
Abstract  

 

Stress response and depression have a crucial impact on modern society. Although the symptoms are well characterized, the molecular mechanisms underlying depression are largely unknown. Currently, the monoaminergic systems, especially serotonergic systems, have received the most attention in the research of depression. Accumulating evidence suggests that the glutamatergic and GABAergic system play an important role in the neurobiology and treatment of this disease. Multiple studies have shown that serotonin (5-HT) could modulate the neurotransmission of glutamic acid (Glu) and gamma-aminobutyric acid (GABA). The orbitofrontal cortex (OFC), which is involved in the pathophysiology and treatment of depression, plays a critical role in the control of higher brain functions and it mainly receives a dense 5-HT innervation from the dorsal raphe nucleus. There exist some 5-HT1A receptors on glutamatergic neurons and GABAergic neurons in the OFC. The purpose of this research was to elucidate the modulatory action of 5-HT1A receptor on the functions of Glu and GABA, which are the principal neurotransmitters mediating excitatory and inhibitory signals in the OFC respectively, in a well-established animal model of depression induced by chronic unpredictable mild stress (CUMS).
We used CUMS in rat to mimic the core symptoms in human. Using the pharmacology approaches by microinjecting of 5-HT1A receptor agonist 8-OH-DPAT and its antagonist WAY100635 to the OFC, we detected behavioral changes by using behavior tests including sucrose preference test, open field test and tail suspension test. In addition, high-performance liquid chromatography (HPLC) was used to detect the level of neurotransmitters such as 5-HT, Glu and GABA in the OFC, respectively.
CUMS group showed a variety of behavioral characteristics of depression, including a significant reduction in the sucrose preference, and locomotion, rearing and grooming in the open field test, and a significant increase in immobility time in the tail suspension test compared with control group. In addition, CUMS group showed that a significant increase in the concentration of Glu, but no significant difference in the levels of 5-HT and GABA in the OFC. Depression-like behaviors of CUMS rats were obviously ameliorated after being treated with 8-OH-DPAT, while the concentration of Glu was decreased. Rats received WAY100635 in control group showed similar depression-like behaviors and similar increased Glu levels. However, 5-HT level and GABA concentration were not altered in both groups received 8-OH-DPAT or WAY100635.

These findings indicated that the mechanism through which CUMS could induce depression-like behaviors, probably is not due to the decrease of 5-HT level within the OFC, but because of excessive release of Glu that resulted from that 5-HT could not regulate glutamatergic neurons or the decrease of 5-HT1A receptor function on glutamatergic neurons within the OFC during stress. Our data suggested that 5-HT1A receptor in the OFC plays an important role in modulating the synthesis and release of Glu during CUMS. This study provided insights on modulation of glutamate transmission in depression.

Keywords 5-hydroxytryptamine      glutamic acid      gamma-aminobutyric acid      depression     
Corresponding Authors: AN Shucheng, E-mail: shuchengan@snnu.edu.cn; 电话:+86-29-85310266   
Issue Date: 25 October 2015
Service
E-mail this article
E-mail Alert
RSS
Articles by authors
LI Jiangna
AN Shucheng
LI Zhen
Cite this article:   
LI Jiangna,AN Shucheng,LI Zhen. Orbitofrontal Cortex 5-HT1A Receptor Modulate Glutamate and GABA in Depression Induced by Chronic Unpredictable Mild Stress[J]. Acta Psychologica Sinica, 10.3724/SP.J.1041.2015.01269
URL:  
http://journal.psych.ac.cn/xlxb/EN/10.3724/SP.J.1041.2015.01269     OR     http://journal.psych.ac.cn/xlxb/EN/Y2015/V47/I10/1269
[1] YU Zengyan; ZHAO Ameng; LIU Aishu. Childhood maltreatment and depression: A Meta-Analysis[J]. Acta Psychologica Sinica, 2017, 49(1): 40-49.
[2] CAO Yanmiao; WANG Meiping; CAO Cong; JI Linqin; ZHANG Wenxin. The interaction between dopamine D2 receptor gene TaqIA polymorphim and peer victimization on early adolescent depression[J]. Acta Psychologica Sinica, 2017, 49(1): 28-39.
[3] REN Zhihong; LI Xianyun; ZHAO Lingbo; YU Xianglian; LI Zhenghan; LAI Lizu; RUAN Yijun; JIANG Guangrong. Effectiveness and mechanism of internet-based self-help intervention for depression: The Chinese version of MoodGYM[J]. Acta Psychologica Sinica, 2016, 48(7): 818-832.
[4] NIU Gengfeng; SUN Xiaojun; ZHOU Zongkui; KONG Fanchang; TIAN Yuan. The impact of social network site (Qzone) on adolescents’ depression: The serial mediation of upward social comparison and self-esteem[J]. Acta Psychologica Sinica, 2016, 48(10): 1282-1291.
[5] CAO Cong; WANG Meiping; JI Linqin; WEI Xing; CAO Yanmiao; ZHANG Wenxin. The MAOA rs6323 polymorphism interacts with maternal supportive parenting in predicting adolescent depression: Testing the diathesis-stress and differential susceptibility hypotheses[J]. Acta Psychologica Sinica, 2016, 48(1): 22-35.
[6] WANG Meiping; ZHANG Wenxin; CHEN Xinyin. The Interaction between rs6295 Polymorphism in the 5-HTR1A Gene and Parenting Behavior on Early Adolescents’ Depression: The Verification of Differential Susceptability Model[J]. Acta Psychologica Sinica, 2015, 47(5): 600-610.
[7] WANG Meiping; JI Linqin; ZHANG Wenxin. Interaction Effects between rs6323 Polymorphism in the MAOA Gene and Peer Relationship on Early Depression among Male Adolescents[J]. Acta Psychologica Sinica, 2015, 47(10): 1260-1268.
[8] JIN Jiafei;XU Shan;WANG Yanxia. A Comparison Study of Role Overload, Work-Family Conflict and Depression between China and North America: The Moderation Effect of Social Support[J]. Acta Psychologica Sinica, 2014, 46(8): 1144-1160.
[9] TANG Mingming;PAN Yuqin;LIN Wenjuan. A New Animal Model of Depression Induced by Repeated Central Lipopolysaccharide Administration[J]. Acta Psychologica Sinica, 2014, 46(5): 639-646.
[10] TIAN Lumei;ZHANG Wenxin;CHEN Guanghui. Effects of Parental Support, Friendship Quality on Loneliness and Depression: To Test An Indirect Effect Model[J]. Acta Psychologica Sinica, 2014, 46(2): 238-251.
[11] WU Shuai;AN Shucheng;CHEN Huibin;LI Fei. Orbital Frontal Cortex D1 Dopamine Receptor Modulate Glutamate and NMDA Receptor in Depression Induced by Chronic Unpredictable Mild Stress[J]. Acta Psychologica Sinica, 2014, 46(1): 69-78.
[12] HONG Xing;XU Xiao-Hong;YANG Yu-Jie;XIE Ling-Dan;WANG Zhen-Lu;ZHANG Qin;ZHANG Guang-Xia;LIU Xing-Yi. Early Life Exposure to Bisphenol A Affects Anxiety and Depression Behaviors in Mice and Its Molecular Mechanism[J]. Acta Psychologica Sinica, 2012, 44(9): 1167-1179.
[13] TIAN Lu-Mei,CHEN Guang-Hui,WANG Shu-Qiong,LIU Hai-Jiao,ZHANG Wen-Xin. Effects of Parental Support and Friendship Support on Loneliness and Depression During Early and Middle Adolescence[J]. , 2012, 44(7): 944-956.
[14] CUI Li-Xia;SHI Guang-Yuan;ZHANG Yu-Jing;YU Yuan. A Study of the Integrated Cognitive Model of Depression for Adolescents and Its Gender Difference[J]. Acta Psychologica Sinica, 2012, 44(11): 1501-1514.
[15] WEN Li-Min;AN Shu-Cheng;LIU Hui. Role of Hippocampal 5-HT1A Receptor and Its Modulation to NMDA Receptor and AMPA Receptor in Depression Induced by Chronic Unpredictable Mild Stress[J]. Acta Psychologica Sinica, 2012, 44(10): 1318-1328.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
Copyright © Acta Psychologica Sinica
Support by Beijing Magtech