ISSN 0439-755X
CN 11-1911/B
主办：中国心理学会
　　　中国科学院心理研究所
出版：科学出版社

心理学报 ›› 2012, Vol. 44 ›› Issue (3): 330-337.

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慢性应激诱导的抑郁大鼠纹状体par-4基因的表达

朱熊兆;彭素芳;张晟;张逸;蔡琳   

  1. (中南大学湘雅二医院心理学研究所, 长沙 410011)
  • 收稿日期:2010-07-17 修回日期:1900-01-01 发布日期:2012-03-28 出版日期:2012-03-28
  • 通讯作者: 朱熊兆

The Expression of Prostate Apoptosis Response-4 in Striatum of A Rat Model of Depression Induced by Chronic Stress

ZHU Xiong-Zhao;PENG Su-Fang;ZHANG Sheng;ZHANG Yi;CAI Lin   

  1. (Medical Psychological Institute, Second Xiangya Hospital, Central South University, Changsha, 410011, China)
  • Received:2010-07-17 Revised:1900-01-01 Online:2012-03-28 Published:2012-03-28
  • Contact: ZHU Xiong-Zhao

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1039

被引次数

5

摘要： 为研究慢性温和应激诱导的抑郁大鼠纹状体内前列腺凋亡反应蛋白(prostate apoptosis response-4, par-4)的表达, 及甲基化是否参与par-4基因表达的调控, 将10周龄大鼠随机分为实验组和对照组, 实验组接受慢性温和应激, 对照组不接受实验性处理。于大鼠13周龄时, 采用强迫游泳、糖水偏爱测验测定大鼠的抑郁水平, 以实时定量PCR检测纹状体par-4及多巴胺D2受体(Dopamine receptor D2, DRD2) mRNA表达水平, 免疫印迹法检测纹状体par-4蛋白质表达水平, 用亚硫酸盐测序法检测par-4基因启动子区甲基化水平。结果发现, 与对照组大鼠相比, 实验组大鼠漂浮时间延长, 糖水偏爱率降低, 脑纹状体par-4、DRD2 mRNA及par-4蛋白质表达水平均降低, par-4基因启动子区甲基化水平两组差异不显著。提示慢性温和应激诱导大鼠产生了抑郁样行为, 并能抑制纹状体par-4基因的表达, 而基因甲基化可能并不参与其调控机制。

关键词: 慢性温和应激, 抑郁样行为, 纹状体, 前列腺凋亡反应蛋白, 基因甲基化

Abstract: Chronic stress may induce depression, but the mechanism is unclear. Previous studies suggested that dopamine system, especially dopamine receptor D2 (DRD2) may be involved in, and prostate apoptosis response-4 (par-4) may be the link point between DRD2 and depression. The aims of this research are to detect the expression of par-4 in striatum of a rat model of depression induced by chronic stress, and to explore whether DNA methylaion is involved in the regulatory mechanism of par-4 expression.
Newborn rats were randomly divided into two groups, when they grew up to ten weeks, the experimental group rats (n = 17) accepted chronic mild stress for 3 weeks, while the control group rats (n = 17) received no experimental handle. Their depressive level was assessed with forced swimming test and sucrose consumption test; the mRNA expression of par-4 and DRD2 in rats’ striatum was detected by Real-Time PCR; the protein expression of par-4 was detected by western blot; and the DNA methylation level of par-4 was investigated by bisulfated DNA sequencing.
The result showed that the float time of experimental rats was longer than the control rats (t = 4.01, p < 0.05), and the sucrose preference rate of experimental rats was reduced compare with the control rats (t = -2.93, p < 0.05). The par-4 and DRD2 mRNA expression of experimental rats were lower than the control rats (t values were -3.56 and -2.507 respectively, p < 0.05), and the fold changes of par-4 and DRD2 mRNA expression in experimental rats compared to control rats were 0.33 and 0.33 respectively. The par-4 protein expression of experimental rats was decreased compared with the control rats (t = -4.92, p < 0.05). However, there was no significant difference between two groups in methylation level of par-4 promoter region (t = -0.26, p > 0.05).
These results suggest that chronic mild stress could induce the depressive behaviors of rats and suppress the expression of par-4, but DNA methylation seems to be not involved in the regulatory mechanism.

Key words: Chronic mild stress, Depressive behaviors, Striatum, Prostate apoptosis response-4, Methylation