ISSN 0439-755X
CN 11-1911/B

心理学报 ›› 2010, Vol. 42 ›› Issue (06): 695-703.

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早期剥夺所致大鼠抑郁样行为与海马BDNF mRNA表达变化


  1. (1暨南大学医学院, 广州 510632) (2广东金融学院劳经系, 广州 510521)
    (3复旦大学社会学博士后流动站, 上海 200433)
  • 收稿日期:2010-02-08 修回日期:1900-01-01 出版日期:2010-06-30 发布日期:2010-06-30
  • 通讯作者: 江雪华

Depression-like Behavior and Change in Hippocampus BDNF mRNA Expression Induced by Early Deprivation in Rats

JIANG Xue-Hua;ZHANG Min;LU Da-Xiang;WEI Jia;QI Ren-Bin   

  1. (1 Medical School, Jinan University, Guangzhou 510632, China)
    (2 Department of Labor Economy and Human Resource Management, Guangdong University of Finance, Guangzhou 510521, China)
    (3 Postdoctoral Mobile Station of Sociology, Fudan University, Shanghai 200433, China)
  • Received:2010-02-08 Revised:1900-01-01 Online:2010-06-30 Published:2010-06-30
  • Contact: JIANG Xue-Hua

摘要: 为探讨早期剥夺对大鼠行为与海马脑源性神经营养因子(BDNF)mRNA表达的影响, 将新生的SD大鼠随机分为正常对照组与早期剥夺组, 早期剥夺组在出生后1~14d每天孤养4h。监测体重, 在出生后36- 64天进行液体消耗试验, 每周一次。12w龄时, 进行穿梭箱试验, 酶联免疫法检测海马BDNF含量, 原位杂交法观察海马BDNF mRNA的表达。早期剥夺组大鼠体重显著低于正常对照组(p<0.01), 糖水摄入量与糖水偏爱显著低于正常对照组(p<0.05或p<0.01)。穿梭箱试验中, 早期剥夺组大鼠在训练时的穿梭反应次数显著少于正常对照组(p<0.05), 在测试时, 两组大鼠的被动逃避行为、主动逃避行为与逃避错误次数无显著差异。早期剥夺组大鼠海马BDNF含量、CA1区和CA3区BDNF mRNA表达显著低于正常对照组(p=0.05或p<0.05)。提示早期剥夺可导致大鼠生长减慢, 表现出快感缺失的抑郁样行为, 但未诱导成年大鼠表现出明显的习得性无助倾向, 早期剥夺能下调成年大鼠海马BDNF mRNA的表达。

关键词: 早期剥夺, 抑郁样行为, 海马, 脑源性神经营养因子

Abstract: There is considerable evidence that adverse early life events have profound and persistent effects on brain functions and may represent a risk factor for the development of depression later in life. Studies of postnatal environmental manipulations in rodents can potentially yield evidence that adverse early-life experience leading to behavior dysfunction is a general mammalian characteristic, and this approach can be used to develop animal models for depression research to explore its neurobiology mechanism.
To investigate the long-term effects of early deprivation (ED) on behavior and expression of brain-derived neurotrophic factor (BDNF) in adult rats, the new-born Sprague-Dawley pups were randomly divided into non-handling (NH) group and ED group. Pups in ED group were isolated from dam, nest and littermates daily for 4 hours from the post-natal day (PND) 1 to PND14 while pups in NH group were undisturbed at room ambient temperature of 21°C. Body weights were monitored after weaning and sucrose preference test was carried out from PND36 to PND64 once a week (n=16). At 12 weeks of age, rats were subjected to behavioral sessions for the foot-shock pre-exposure/shuttle box test (n=8), the contents of BDNF in hippocampus were measured by enzyme-linked immunosorbent assay (n=8) and hippocampus BDNF mRNA expression was detected by in situ hybridization (n=6).
Body weights of ED rats were significantly lower than NH rats (p<0.01), sucrose intake and sucrose preference were significantly decreased in ED rats compared with NH rats (p<0.05 or p<0.01). In contrast to NH rats, ED rats performed less barrier crossings in the training session of shuttle box test (p<0.05), but in the testing session, comparison of the manipulation groups yielded no significant differences. The contents of BDNF in hippocampus and the BDNF mRNA expression in CA1and CA3 of ED rats were decreased significantly (p<0.05 or p<0.01).
The results suggest that ED may result in the decrease of growth, the depression-like behavior of anhedonia, and the reduction of hippocampus BDNF mRNA expression in Sprague-Dawley rats, but did not induce the behavior of learned helplessness in a foot-shock pre-exposure/shuttle box test. The reduction of BDNF expression in hippocampus may play a role in the development of depression-like behavior induced by ED.

Key words: early deprivation, depression-like behavior, hippocampus, brain-derived neurotrophic factor (BDNF)