ISSN 0439-755X
CN 11-1911/B
主办:中国心理学会
   中国科学院心理研究所
出版:科学出版社

心理学报 ›› 2003, Vol. 35 ›› Issue (02): 261-265.

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条件反射性抗体反应增强的动态分析——以OVA为非条件刺激物

陈极寰,林文娟,王玮雯,杨杰,邵枫   

  1. 中国科学院心理研究所脑-行为研究中心,北京 100101
  • 收稿日期:2002-10-03 修回日期:1900-01-01 出版日期:2003-03-30 发布日期:2003-03-30
  • 通讯作者: 林文娟

THE DYNAMIC ANALYSIS OF CONDITIONED ENHANCEMENT OF ANTIBODY RESPONSE TO OVALBUMIN

Chen Jihuan, Lin Wenjuan, Wang Weiwen, Yang Jie, Shao Feng   

  1. Brain-Behavior Research Center, Institute of Psychology, Chinese Academy of Sciences, Beijing, 100101
  • Received:2002-10-03 Revised:1900-01-01 Published:2003-03-30 Online:2003-03-30
  • Contact: Lin Wenjuan

摘要: 研究了条件反射性抗体反应增强模型的建立。被试为49只雄性成年Wistar大鼠,采用糖精水作为条件性刺激,一种蛋白抗原卵清蛋白作为非条件性刺激配对给予大鼠,两者结合后,在初次抗体应答下降阶段再次单独给予条件刺激,用酶联免疫吸附法分时段检测抗体水平的变化。发现条件组在条件刺激后15,20,25天左右抗体水平明显高于对照组。这一过程与初次抗体应答的规律类似。这些结果证实经一次条件训练,单独给予条件刺激能诱导出明显的条件反射性抗体反应增高。

关键词: 条件反射性免疫增强, 动态分析, 卵清蛋白, 大鼠

Abstract: To establish the model of conditioned enhancement of antibody production. Forty-nine male and adult Wistar rats were used. A novel taste of saccharin was served as the conditioned stimulus (CS) and an injection of a protein antigen ovalbumin as the unconditioned stimulus (UCS). After a CS/UCS pairing was made, animals were re-exposed to the CS alone. Blood samples were periodically collected by tail incisions after re-exposure to the CS. The level of anti-OVA antibody production was measured by enzyme linked immunosorbent assay (ELISA). The results showed that the levels of anti-OVA antibody production of the group CS were significantly greater than the controls at the day 15, 20 and 25 after the CS presentation. The pattern of the conditioned anti-OVA IgG production was similar to that of the primary antibody response. The results reinforced the view that the enhancement of antibody production can be induced by single-trial Pavlovian conditioning where the CS alone is sufficient.

Key words: conditioned immunoenhancement, dynamic analysis, rat, ovalbumin

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